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High-dose Vitamin B6 supplements may improve anxiety and mood - clinical trial

University of Reading

supplements - Credit Pixabay CC0 public domain

Scientists at the University of Reading measured the impact of high doses of Vitamin B6 on young adults and found that they reported feeling less anxious and depressed after taking the supplements every day for a month.


High-dose Vitamin B6 supplementation for one month improved self-ratings of anxiety and mood in young adults compared with placebo treatment in this randomised controlled trial.

The study also provided evidence suggesting that these effects could reflect increased levels of GABA - a major 'inhibitory' neurotransmitter - as the B6 supplementation also led to (harmless) changes in performance on a visual test known to be sensitive to GABA levels.
No significant effects were seen in a third group who were randomised to receive high dose Vitamin B12 supplements.

While these findings are consistent with some previous reports that B6 supplementation can help in some mental health conditions, it should be emphasised that:

  • the doses of B6 used in this trial were extremely high, i.e. at pharmaceutical levels not obtainable from food, and not considered safe for long-term use without medical supervision (100mg/day was provided, which is hugely in excess of the Recommended Daily Intake for young adults of 1.3mg, and also exceeds the EU Upper Tolerable Intake level for long-term use, of 25mg/day)
  • the participants were generally healthy university students, so these findings cannot be generalised to anyone suffereing from clinically diagnosed anxity or depression  
  • no measures of the participants' actual Vitamin B6 status were reported before or after treatment

For these reasons, these findings seem unlikely to have any immediate relevance for the actual treatment of anxiety - although they do make a useful new and interesting contribution to the scientific evidence base concerning Vitamin B6, and its importance for mental as well as physical health.  

For more details of the research, see:

For more information on Vitamin B6 and brain function, see also:

The study, published in the journal Human Psychopharmacology: Clinical and Experimental, provides valuable evidence to support the use of supplements thought to modify levels of activity in the brain for preventing or treating mood disorders.

Dr. David Field, lead author from the School of Psychology and Clinical Language Sciences at the University of Reading, said: "The functioning of the brain relies on a delicate balance between the excitatory neurons that carry information around and inhibitory ones, which prevent runaway activity.

"Recent theories have connected mood disorders and some other neuropsychiatric conditions with a disturbance of this balance, often in the direction of raised levels of brain activity.

"Vitamin B6 helps the body produce a specific chemical messenger that inhibits impulses in the brain, and our study links this calming effect with reduced anxiety among the participants."

While previous studies have produced evidence that multivitamins or marmite can reduce stress levels, few studies have been carried out into which particular vitamins contained within them drive this effect.

The new study focused on the potential role of Vitamins B6, which is known to increase the body's production of GABA (Gamma-Aminobutyric Acid), a chemical that blocks impulses between nerve cells in the brain.

In the current trial, more than 300 participants were randomly assigned either Vitamin B6 or B12 supplements far above the recommended daily intake (approximately 50 times the recommended daily allowance) or a placebo, and took one a day with food for a month.

The study showed that Vitamin B12 had little effect compared to placebo over the trial period, but Vitamin B6 made a statistically reliable difference.

Raised levels of GABA among participants who had taken Vitamin B6 supplements were confirmed by visual tests carried out at the end of the trial, supporting the hypothesis that B6 was responsible for the reduction in anxiety. Subtle but harmless changes in visual performance were detected, consistent with controlled levels of brain activity.

Dr. Field said: "Many foods, including tuna, chickpeas and many fruits and vegetables, contain Vitamin B6. However, the high doses used in this trial suggest that supplements would be necessary to have a positive effect on mood.

"It is important to acknowledge that this research is at an early stage and the effect of Vitamin B6 on anxiety in our study was quite small compared to what you would expect from medication. However, nutrition-based interventions produce far fewer unpleasant side effects than drugs, and so in the future people might prefer them as an intervention.

"To make this a realistic choice, further research is needed to identify other nutrition-based interventions that benefit mental wellbeing, allowing different dietary interventions to be combined in future to provide greater results.

"One potential option would be to combine Vitamin B6 supplements with talking therapies such as Cognitive Behavioural Therapy to boost their effect."